Tsuyoshi, Imai and Taroepratjeka, Dyah Asri Handayani and Prapaipid, Chairattanamanokorn and Alissara, Reungsang (2019) Microbial community analysis of extremely halophilic bacteria from a salt pan and salt damaged soil in Thailand dor biohydrogen production from lignocellulosic biomass. In: CESE 2019 : The twelfth annual conference on the chalenges in environmental science and engineering, 3-7 November 2019, Kaoshiung, Taiwan.
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Abstract
Alkaline pretreatment with NaOH is considered the most cost-effective method for biohydrogen production from lignocellulosic biomass. However, a considerable amount of water is needed to wash Na+ions before continuing to the next process. Extremely halophilic bacteria can help reduce water usage owing to their ability to produce hydrogen under conditions of high NaCI concentrations. The aim of this study was to find out extremely halophilic bacteria genus suitable for biohydrogen production under nearly saturated NaCI condition. Three soil samples from a salt pan and salt damaged soil have been cultivated and acclimatized in 26% (w/w) of NaCI to screen for biohydrogen producing extremely halophilic bacteria. High-throughput sequencing of V3-V4 regions of 16s rRNA amplicons with average length of 428bp, average total of 113.620 sequences reads, and average OTUs of 115, revealed tha Halanaerobium sp, was the predominat genus in two of the samples (97-98%), while halanaerobacter sp, was predominant in the other (61%). Biohydrogen production experiments with inoculums from those acclimatized samples gave hydrogen molar yields of 1.20 and 1.08mol H2/mol glucose in serum bottles with inoculum gave a lower yield of 0.78 mol h2/mol glucose at 37’C. the results from this study showed tha inoculums predominant with Halanaerobium sp, were able to produce higher biohydrogen yield compared to inoculum predominant with Halanaerobacter sp, under nearly saturated NaCI condition.
| Item Type: | Conference or Workshop Item (Paper) |
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| Subjects: | T Technology > TD Environmental technology. Sanitary engineering |
| Divisions: | Paper |
| Depositing User: | Azizullah Putri Akbar |
| Date Deposited: | 02 Mar 2022 08:48 |
| Last Modified: | 02 Mar 2022 08:48 |
| URI: | http://eprints.itenas.ac.id/id/eprint/1903 |
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