Taroepratjeka, Dyah Asri Handayani and Tsuyoshi, Imai (2018) Microbial community characteristics of halotolerant hydrogen producing bacteria from salt pan and salt damaged soil in Thailand. In: Seminar Park, Yamaguchi, Japan, 13-14 November 2018, Jepang.

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Abstract

Biohydrogen production has been extensively studied by researchers all over the world for its promising use an alternative energy source. Previous studies have shown that biohydrogen production from lignocellulosic biomass was possible with the aid of bachteria. But the process requires pretreatment with alkaline (NaOH) and heat, and also enzyme hydrolysis befor the bacteria can digest the cellulosic biomass. The high concentration of NaOH after the processs makes it unfavorable for most of bacteria to live. However, halotolerant bacteria can survive in high salinity condition where as this condition increase osmotic pressure and provide inhibition to most other bacteria’s activity. The bacteria in this study have been cultivated from soil samples of salt damaged soil in Khon Kaen, Thailand, and Commecial salt pan field near Bangkok, Thailand and kept at anaerobic saturated (26%) sodium condition for three years. These halotolerant bacteria are capable to produce hydrogen under saturated salt (NaCI) condition without methane (CH4) by- products. In this study, we compared the difference of the microbial community characteristics of the original soil samples and the acclimatized cultures which have bee kept at saturated condition. The characteristization of the microbial community was using 16-rRNA-based molecular technique, including Polymerase Chain Reaction-based Denaturing Gradient Gel Electrophoresis (PCR-DGGE) and sequaencing. PCR-DGGE has confirmed distinguish band pattern differences between the original soil samples and acclimatized cultures.

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