Taroepratjeka, Dyah Asri Handayani and Tsuyoshi, Imai (2017) Bio-hydrogen Production from Cellulosic Biomass by Extremely Salt Tolerant Bacteria. In: The 13th Young Scientist Seminar, November 18th-19th, 2017, Yamaguchi University, Japan.

Preview

Text B8 Bio-hydrogen production from cellulosic biomass.pdf Download (2MB) | Preview

Abstract

Fossil fuel energy crisis has led a significant interest for clean alternative energy sources. Energy production from biomass especially has gained a renewed interest because it's readily available and allow a cost-effective use. Previous studies have shown that bio-hydrogen production from cellulosic biomass was possible with the application of anaerobic bacteria. But the process requires pretreatment with alkaline (NaOH) and heat, and also enzyme hydrolysis before the anaerobic bacteria can digest the cellulosic biomass. The high concentration of NaOH after the pretreatment process makes it difficult for most of bacteria to live. However, extremely salt tolerant bacteria (hulophile) can survrve in high salinity condition, whereas this condition increase osmotic pressure and provide inhibition to most other bacteria's activity. The hypothesis is that the extremely salt tolerant hydrogen producing bacteria can be tolerant to high concentration of sodium ion. Extremely salt tolerant bacteria have adapted their cytoplasm osmotically with their high salt concentration medium. The bacteria in this study have been cultivated from soil samples of salt damaged soil in Khon Kaen. 'fhailand, and commercial salt pan field near Bangkok, Thailand and kept at anaerobic saturated sodiurn condition (26%) and incubation temperature of 37oC in 500 mL cultivation serum bottles. These extremely salt tolerant bacteria are capable to produce hyd'cgen under saturated salt (NaCl) condition without methane (CHr) byproducts. To examine this hypothesis, first, we will conduct an experiment to investigate what kind(s) of the environmental fbctor that makes the extremely salt tolerant bacteria's high activity in high salinity condition, especially, whether it's mostly the sodium ions. chloride ions, or interaction of both. This step is necessary to customize the following pretreatment processes. The experiment will be done in 75 ml serum bottles in duplicates, and will be kept in shaking water bath to ensure oprimum conditions. We will also acclimatize the bacteria from pure D-glucose carbon source during the cultivation to other different carbon sources which are most likely will be produced from cellulosic biomass hydrolysis, such as xylose and arabinose. After that, hydrogen production process from cellulose will be developed. For this experiment, we will use wooden disposable chopstick waste from Faculty of Engineering's Cafeteria in Yamaguchi University as the cellulosic biomass source. Finally, we will screen and characterize the microbial community by using 16-rRNA-based molecular techniques, including Polymerase Chain Reaction u,ith based Denaturing Gradient Gel Electrophoresis (PCR-DGGE) and sequencing.

Actions (login required)

View Item